Isoproterenol and cAMP Block ERK Phosphorylation and Enhance [Ca]i Increases and Oxygen Consumption by Muscarinic Receptor Stimulation in Rat Parotid and Submandibular Acinar Cells
نویسنده
چکیده
Isoproterenol and cAMP Block ERK Phosphorylation and Enhance [Ca]i Increases and Oxygen Consumption by Muscarinic Receptor Stimulation in Rat Parotid and Submandibular Acinar Cells Stephen P. Soltoff and Lee Hedden From Division of Signal Transduction, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, 02115 Running head: Isoproterenol blocks ERK phosphorylation by M3R, P2X7R, and EGFR Address correspondence to: Stephen P. Soltoff, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, EC/CLS-406, Boston, MA 02215. PHONE: 617-735-2640; FAX: 617-735-2646; E-mail: [email protected]
منابع مشابه
Consumption by Muscarinic Receptor Stimulation in Rat Parotid and Submandibular Acinar Cells
Isoproterenol and cAMP Block ERK Phosphorylation and Enhance [Ca]i Increases and Oxygen Consumption by Muscarinic Receptor Stimulation in Rat Parotid and Submandibular Acinar Cells Stephen P. Soltoff and Lee Hedden From Division of Signal Transduction, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, 02115 Running head: Isoproterenol blocks ERK phosphorylatio...
متن کاملIsoproterenol and cAMP block ERK phosphorylation and enhance [Ca2+]i increases and oxygen consumption by muscarinic receptor stimulation in rat parotid and submandibular acinar cells.
Salivary glands are innervated by sympathetic and parasympathetic neurons, which release neurotransmitters that promote fluid secretion and exocytosis when they bind to muscarinic and beta-adrenergic receptors, respectively. Signaling pathways downstream of these receptors are mainly distinct, but there is cross-talk that affects receptor-dependent events. Here we report that the beta-adrenergi...
متن کاملBeta-adrenergic receptor stimulation induces inositol trisphosphate production and Ca2+ mobilization in rat parotid acinar cells.
In dispersed rat parotid gland acinar cells, the beta-adrenergic agonist (-)-isoproterenol, but not its stereoisomer (+)-isoproterenol, induced a transient 1.6-fold (at maximum stimulation, 2 x 10(-4) M) increase in cytosolic free calcium ([Ca2+]i) within 9 s, which returned to resting levels (approximately 190 nM) by 60 s. This [Ca2+]i response was not altered by chelating extracellular Ca2+ w...
متن کاملSuppression of carbachol-induced oscillatory Cl- secretion by forskolin in rat parotid and submandibular acinar cells.
Sympathetic stimulation induces weak salivation compared with parasympathetic stimulation. To clarify this phenomenon in salivary glands, we investigated cAMP-induced modulation of Ca(2+)-activated Cl(-) secretion from rat parotid and submandibular acinar cells because fluid secretion from salivary glands depends on the Cl(-) secretion. Carbachol (Cch), a Ca(2+)-increasing agent, induced hyperp...
متن کاملInvolvement of phosphodiesterase 4 in β-adrenoceptor agonist-induced amylase release in parotid acinar cells
β-Adrenoceptor activation increases intracellular cAMP levels and consequently induces exocytotic amylase release in parotid acinar cells. Phosphodiesterase (PDE) catalyses the hydrolysis of cAMP, which terminates the downstream signaling of this second messenger. We investigated the involvement of PDE4, a cAMP-PDE, in β-adrenoceptor agonistinduced amylase release in mouse, rat and rabbit parot...
متن کامل